Check out the new Thesis page – with full PDFs of the 7 PhD thesis from our lab

Lab News

  • Nov 2018: Tom will be giving talks at Chulalongkorn University and VISTEC Institutes in Thailand and at the 7th IYSGC meeting in Sydney, Australia
  • Oct 2018: We’ve now published our work on design of modular hairpin structures for tuning yeast translation tuning yeast translation
  • Sep 2018: Glen’s work on automating yeast multigene assembly with the London DNA Foundry is published in SLAS Technology
  • July 2018: Tom is back in London in time to see Charlie successfully defend his PhD thesis – congrats Dr Gilbert!
  • May 2018: Olivier’s paper on burden prediction using cell lysate measurements is published in Nature Communications
  • Apr 2018; Francesca and Alice’s paper on Burden-driven feedback control of gene expression is published in Nature Methods.
  • Mar 2018; George’s two papers on engineering yeast filamentous growth switching and inducible mother-specific expression are published in Communications Biology  and PLoS One.
  • Nov 2017; Charlie and Rodrigo will both be presenting at SBUK 2017 in Manchester
  • Oct 2017; Tom and Olivier will both be presenting at CRI Paris’ 10Y iGEM event
  • Aug 2017; Our latest work on burden is up on BioRxiv – exciting preprints from Olivier and Francesca

Announcements

Two major new papers are now out from our lab’s collaborations with Guy-Bart Stan on the key issue of gene expression costs in bacteria. Both build on our 2015 paper on burden and were recently covered in an Imperial College London news article.

Firstly, published in Nature Methods is an RNAseq study on the immediate effects of burden in E. coli. This study led to the creation of a general and tunable dCas9-based feedback controller to automatically control expression levels to tolerable amounts for cell growth. This work was led by Francesca Ceroni during her time with our group, with key contributions from our student Alice Boo and from Simone Furini in Italy and Tom Gorochowski from Bristol.

Olivier Borkowski from our group was also involved in that paper, but additionally has his own work on burden now published in Nature Communications. In this paper, cell lysate technology is used to measure the burden of making a protein and this data feeds a model to predict how expressing it would decrease the growth of E. coli. This can even be used to predict multigene and operon expression burden, and we hope will become a widely-used approach.

Both Francesca and Olivier have now moved to their own junior research fellow positions, with Francesca based at Chemical Engineering at Imperial and Olivier returning to Paris to join Genopole.