Collaborators: Jef Boeke (NYU), Yizhi Cai (Manchester), Steve Oliver (Cam), Paul Freemont, Sc2.0 Consortium
Sc2.0 is a high-profile, international project to do the first full synthesis of a eukaryotic cell genome, the model yeast species Saccharomyces cerevisiae. Led by Prof Jef Boeke at NYU, USA, an international consortium is now established to re-synthesis and make to design changes to all 16 yeast chromosomes. Our group is leading the UK effort in the consortium, with the design, synthesis and assembly of the complete 666 kbp chromosome XI. During construction we will investigate DNA assembly methods and genome design and topology and learn how these can be used to optimise gene expression in metabolic engineering experiments.
Funders: BBSRC, EPSRC